Construct: shRNA TRCN0000180821

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024510.2-1659s1c1
DNA Barcode:
GCAGTGGTTCATGCCAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYBC1 (79415)
Gene Description:
cytochrome b-245 chaperone 1
Transcript:
RefSeq NM_024510.2 (NON-CURRENT)
Match location:
Position 1659 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180821)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGGTTCATGCCAGTAAT
Hairpin Sequence:
5'-CCGG-GCAGTGGTTCATGCCAGTAAT-CTCGAG-ATTACTGGCATGAACCACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGGTTCATGCCAGTAATCTCGAGATTACTGGCATGAACCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGGTTCATGCCAGTAATCTCGAGATTACTGGCATGAACCACTGC-3'

Other clones with same target sequence:

(none)