Construct: shRNA TRCN0000180834

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172824.1-909s1c1
DNA Barcode:
GCTGGATCTTACGAGTTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc14 (239839)
Gene Description:
coiled-coil domain containing 14
Transcript:
RefSeq NM_172824.1 (NON-CURRENT)
Match location:
Position 909 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180834)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATCTTACGAGTTGTAT
Hairpin Sequence:
5'-CCGG-GCTGGATCTTACGAGTTGTAT-CTCGAG-ATACAACTCGTAAGATCCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATCTTACGAGTTGTATCTCGAGATACAACTCGTAAGATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATCTTACGAGTTGTATCTCGAGATACAACTCGTAAGATCCAGC-3'

Other clones with same target sequence:

(none)