Construct: shRNA TRCN0000180983

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027878.1-705s1c1
DNA Barcode:
CAAGCTGGAATGGAATCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dram1 (71712)
Gene Description:
DNA-damage regulated autophagy modulator 1
Transcript:
RefSeq NM_027878.1 (NON-CURRENT)
Match location:
Position 705 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180983)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGCTGGAATGGAATCCAAA
Hairpin Sequence:
5'-CCGG-CAAGCTGGAATGGAATCCAAA-CTCGAG-TTTGGATTCCATTCCAGCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGCTGGAATGGAATCCAAACTCGAGTTTGGATTCCATTCCAGCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGCTGGAATGGAATCCAAACTCGAGTTTGGATTCCATTCCAGCTTG-3'

Other clones with same target sequence:

(none)