Construct: shRNA TRCN0000181041

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152336.1-902s1c1
DNA Barcode:
CAAGTGCGTGAGTTCGAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGBL1 (123624)
Gene Description:
ATP/GTP binding protein like 1
Transcript:
RefSeq NM_152336.1 (NON-CURRENT)
Match location:
Position 902 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181041)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTGCGTGAGTTCGAGTAT
Hairpin Sequence:
5'-CCGG-CAAGTGCGTGAGTTCGAGTAT-CTCGAG-ATACTCGAACTCACGCACTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTGCGTGAGTTCGAGTATCTCGAGATACTCGAACTCACGCACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTGCGTGAGTTCGAGTATCTCGAGATACTCGAACTCACGCACTTG-3'

Other clones with same target sequence:

(none)