Construct: shRNA TRCN0000181080

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004065.3-431s1c1
DNA Barcode:
CGCTATCTAGATGAGGTGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKAP2 (11217)
Gene Description:
A-kinase anchoring protein 2
Transcript:
RefSeq NM_001004065.3 (NON-CURRENT)
Match location:
Position 431 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181080)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTATCTAGATGAGGTGCTA
Hairpin Sequence:
5'-CCGG-CGCTATCTAGATGAGGTGCTA-CTCGAG-TAGCACCTCATCTAGATAGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTATCTAGATGAGGTGCTACTCGAGTAGCACCTCATCTAGATAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTATCTAGATGAGGTGCTACTCGAGTAGCACCTCATCTAGATAGCG-3'

Other clones with same target sequence:

(none)