Construct: shRNA TRCN0000181098

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026872.1-1581s1c1
DNA Barcode:
CAGCTTCCTAACATGACTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ubap2 (68926)
Gene Description:
ubiquitin-associated protein 2
Transcript:
RefSeq NM_026872.1 (CURRENT)
Match location:
Position 1581 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181098)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTTCCTAACATGACTGTT
Hairpin Sequence:
5'-CCGG-CAGCTTCCTAACATGACTGTT-CTCGAG-AACAGTCATGTTAGGAAGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTTCCTAACATGACTGTTCTCGAGAACAGTCATGTTAGGAAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTTCCTAACATGACTGTTCTCGAGAACAGTCATGTTAGGAAGCTG-3'

Other clones with same target sequence:

(none)