Construct: shRNA TRCN0000181110

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177175.2-1551s1c1
DNA Barcode:
CCTGGTCTTTGGTTTCATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem215 (320500)
Gene Description:
transmembrane protein 215
Transcript:
RefSeq NM_177175.2 (NON-CURRENT)
Match location:
Position 1551 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181110)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTCTTTGGTTTCATGTT
Hairpin Sequence:
5'-CCGG-CCTGGTCTTTGGTTTCATGTT-CTCGAG-AACATGAAACCAAAGACCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTCTTTGGTTTCATGTTCTCGAGAACATGAAACCAAAGACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTCTTTGGTTTCATGTTCTCGAGAACATGAAACCAAAGACCAGG-3'

Other clones with same target sequence:

(none)