Construct: shRNA TRCN0000181167

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023633.1-1632s1c1
DNA Barcode:
CGAGTTTATCACCTAGAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Riox1 (71952)
Gene Description:
ribosomal oxygenase 1
Transcript:
RefSeq NM_023633.1 (NON-CURRENT)
Match location:
Position 1632 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181167)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGTTTATCACCTAGAGGAA
Hairpin Sequence:
5'-CCGG-CGAGTTTATCACCTAGAGGAA-CTCGAG-TTCCTCTAGGTGATAAACTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGTTTATCACCTAGAGGAACTCGAGTTCCTCTAGGTGATAAACTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGTTTATCACCTAGAGGAACTCGAGTTCCTCTAGGTGATAAACTCG-3'

Other clones with same target sequence:

(none)