Construct: shRNA TRCN0000181216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175430.2-2841s1c1
DNA Barcode:
CCTGGTGACACATCTTTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc40 (207607)
Gene Description:
coiled-coil domain containing 40
Transcript:
RefSeq NM_175430.2 (NON-CURRENT)
Match location:
Position 2841 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTGACACATCTTTCAAA
Hairpin Sequence:
5'-CCGG-CCTGGTGACACATCTTTCAAA-CTCGAG-TTTGAAAGATGTGTCACCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTGACACATCTTTCAAACTCGAGTTTGAAAGATGTGTCACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTGACACATCTTTCAAACTCGAGTTTGAAAGATGTGTCACCAGG-3'

Other clones with same target sequence:

(none)