Construct: shRNA TRCN0000181222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026529.2-389s1c1
DNA Barcode:
CCTGTAGAACATGCAACAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2700062C07Rik (68046)
Gene Description:
RIKEN cDNA 2700062C07 gene
Transcript:
RefSeq NM_026529.2 (NON-CURRENT)
Match location:
Position 389 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTAGAACATGCAACAGAA
Hairpin Sequence:
5'-CCGG-CCTGTAGAACATGCAACAGAA-CTCGAG-TTCTGTTGCATGTTCTACAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTAGAACATGCAACAGAACTCGAGTTCTGTTGCATGTTCTACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTAGAACATGCAACAGAACTCGAGTTCTGTTGCATGTTCTACAGG-3'

Other clones with same target sequence:

(none)