Construct: shRNA TRCN0000181229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173784.3-615s1c1
DNA Barcode:
CACAGACACAGTGTTTCACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ubtd2 (327900)
Gene Description:
ubiquitin domain containing 2
Transcript:
RefSeq NM_173784.3 (CURRENT)
Match location:
Position 615 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAGACACAGTGTTTCACAT
Hairpin Sequence:
5'-CCGG-CACAGACACAGTGTTTCACAT-CTCGAG-ATGTGAAACACTGTGTCTGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAGACACAGTGTTTCACATCTCGAGATGTGAAACACTGTGTCTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAGACACAGTGTTTCACATCTCGAGATGTGAAACACTGTGTCTGTG-3'

Other clones with same target sequence:

(none)