Construct: shRNA TRCN0000181501

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024201.2-750s1c1
DNA Barcode:
GCTCATGTGGATGTATCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc127 (67433)
Gene Description:
coiled-coil domain containing 127
Transcript:
RefSeq NM_024201.2 (NON-CURRENT)
Match location:
Position 750 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181501)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATGTGGATGTATCTCAA
Hairpin Sequence:
5'-CCGG-GCTCATGTGGATGTATCTCAA-CTCGAG-TTGAGATACATCCACATGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATGTGGATGTATCTCAACTCGAGTTGAGATACATCCACATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATGTGGATGTATCTCAACTCGAGTTGAGATACATCCACATGAGC-3'

Other clones with same target sequence:

(none)