Construct: shRNA TRCN0000181509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029816.1-247s1c1
DNA Barcode:
GAGTGCTTTGAGGAGAAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2610028H24Rik (76964)
Gene Description:
RIKEN cDNA 2610028H24 gene
Transcript:
RefSeq NM_029816.1 (NON-CURRENT)
Match location:
Position 247 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGCTTTGAGGAGAAGGAA
Hairpin Sequence:
5'-CCGG-GAGTGCTTTGAGGAGAAGGAA-CTCGAG-TTCCTTCTCCTCAAAGCACTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGCTTTGAGGAGAAGGAACTCGAGTTCCTTCTCCTCAAAGCACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGCTTTGAGGAGAAGGAACTCGAGTTCCTTCTCCTCAAAGCACTC-3'

Other clones with same target sequence:

(none)