Construct: shRNA TRCN0000181593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198861.1-454s1c1
DNA Barcode:
GATCACACACGACCTCATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrc75a (192976)
Gene Description:
leucine rich repeat containing 75A
Transcript:
RefSeq NM_198861.1 (CURRENT)
Match location:
Position 454 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCACACACGACCTCATCAT
Hairpin Sequence:
5'-CCGG-GATCACACACGACCTCATCAT-CTCGAG-ATGATGAGGTCGTGTGTGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCACACACGACCTCATCATCTCGAGATGATGAGGTCGTGTGTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCACACACGACCTCATCATCTCGAGATGATGAGGTCGTGTGTGATC-3'

Other clones with same target sequence:

(none)