Construct: shRNA TRCN0000181609

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144901.2-2796s1c1
DNA Barcode:
GACCAGATAACTCAATGGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csde1 (229663)
Gene Description:
cold shock domain containing E1, RNA binding
Transcript:
RefSeq NM_144901.2 (NON-CURRENT)
Match location:
Position 2796 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181609)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCAGATAACTCAATGGGAT
Hairpin Sequence:
5'-CCGG-GACCAGATAACTCAATGGGAT-CTCGAG-ATCCCATTGAGTTATCTGGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCAGATAACTCAATGGGATCTCGAGATCCCATTGAGTTATCTGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCAGATAACTCAATGGGATCTCGAGATCCCATTGAGTTATCTGGTC-3'

Other clones with same target sequence:

  1. TRCN0000319938