Construct: shRNA TRCN0000181668

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172428.1-239s1c1
DNA Barcode:
GAATGACATCCACCAGCAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc134 (76457)
Gene Description:
coiled-coil domain containing 134
Transcript:
RefSeq NM_172428.1 (NON-CURRENT)
Match location:
Position 239 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181668)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGACATCCACCAGCAGTA
Hairpin Sequence:
5'-CCGG-GAATGACATCCACCAGCAGTA-CTCGAG-TACTGCTGGTGGATGTCATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGACATCCACCAGCAGTACTCGAGTACTGCTGGTGGATGTCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGACATCCACCAGCAGTACTCGAGTACTGCTGGTGGATGTCATTC-3'

Other clones with same target sequence:

(none)