Construct: shRNA TRCN0000181711

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172492.2-2016s1c1
DNA Barcode:
GATCAGAAGCAGATCTCGTCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrtm2 (211187)
Gene Description:
leucine-rich repeats and transmembrane domains 2
Transcript:
RefSeq NM_172492.2 (NON-CURRENT)
Match location:
Position 2016 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181711)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAGAAGCAGATCTCGTCT
Hairpin Sequence:
5'-CCGG-GATCAGAAGCAGATCTCGTCT-CTCGAG-AGACGAGATCTGCTTCTGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAGAAGCAGATCTCGTCTCTCGAGAGACGAGATCTGCTTCTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAGAAGCAGATCTCGTCTCTCGAGAGACGAGATCTGCTTCTGATC-3'

Other clones with same target sequence:

(none)