Construct: shRNA TRCN0000181782

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177901.2-666s1c1
DNA Barcode:
GCTCCCGACAACAACTATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4933402J07Rik (330820)
Gene Description:
RIKEN cDNA 4933402J07 gene
Transcript:
RefSeq NM_177901.2 (NON-CURRENT)
Match location:
Position 666 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181782)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCCGACAACAACTATGAA
Hairpin Sequence:
5'-CCGG-GCTCCCGACAACAACTATGAA-CTCGAG-TTCATAGTTGTTGTCGGGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCCGACAACAACTATGAACTCGAGTTCATAGTTGTTGTCGGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCCGACAACAACTATGAACTCGAGTTCATAGTTGTTGTCGGGAGC-3'

Other clones with same target sequence:

(none)