Construct: shRNA TRCN0000181790

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009075.1-865s1c1
DNA Barcode:
GTCTACTTTGGGATGCAGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpia (19895)
Gene Description:
ribose 5-phosphate isomerase A
Transcript:
RefSeq NM_009075.1 (NON-CURRENT)
Match location:
Position 865 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181790)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTACTTTGGGATGCAGGAT
Hairpin Sequence:
5'-CCGG-GTCTACTTTGGGATGCAGGAT-CTCGAG-ATCCTGCATCCCAAAGTAGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTACTTTGGGATGCAGGATCTCGAGATCCTGCATCCCAAAGTAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTACTTTGGGATGCAGGATCTCGAGATCCTGCATCCCAAAGTAGAC-3'

Other clones with same target sequence:

  1. TRCN0000328576