Construct: shRNA TRCN0000181841

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026675.1-199s1c1
DNA Barcode:
GCCCAACCTTGGATCTTTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nudt22 (68323)
Gene Description:
nudix (nucleoside diphosphate linked moiety X)-type motif 22
Transcript:
RefSeq NM_026675.1 (NON-CURRENT)
Match location:
Position 199 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181841)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAACCTTGGATCTTTGAT
Hairpin Sequence:
5'-CCGG-GCCCAACCTTGGATCTTTGAT-CTCGAG-ATCAAAGATCCAAGGTTGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAACCTTGGATCTTTGATCTCGAGATCAAAGATCCAAGGTTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAACCTTGGATCTTTGATCTCGAGATCAAAGATCCAAGGTTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000328052