Construct: shRNA TRCN0000181889

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181418.2-1153s1c1
DNA Barcode:
GCAGTATAGTGAGGACTGTGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ushbp1 (234395)
Gene Description:
USH1 protein network component harmonin binding protein 1
Transcript:
RefSeq NM_181418.2 (NON-CURRENT)
Match location:
Position 1153 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181889)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTATAGTGAGGACTGTGA
Hairpin Sequence:
5'-CCGG-GCAGTATAGTGAGGACTGTGA-CTCGAG-TCACAGTCCTCACTATACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTATAGTGAGGACTGTGACTCGAGTCACAGTCCTCACTATACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTATAGTGAGGACTGTGACTCGAGTCACAGTCCTCACTATACTGC-3'

Other clones with same target sequence:

(none)