Construct: shRNA TRCN0000181893

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025506.1-250s1c1
DNA Barcode:
GCAGTTGCGAGATTTCAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Riiad1 (66353)
Gene Description:
regulatory subunit of type II PKA R-subunit (RIIa) domain containing 1
Transcript:
RefSeq NM_025506.1 (NON-CURRENT)
Match location:
Position 250 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181893)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTGCGAGATTTCAAGAT
Hairpin Sequence:
5'-CCGG-GCAGTTGCGAGATTTCAAGAT-CTCGAG-ATCTTGAAATCTCGCAACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTGCGAGATTTCAAGATCTCGAGATCTTGAAATCTCGCAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTGCGAGATTTCAAGATCTCGAGATCTTGAAATCTCGCAACTGC-3'

Other clones with same target sequence:

(none)