Construct: shRNA TRCN0000181915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026844.2-169s1c1
DNA Barcode:
CCTGCTAAAGGAATGTCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cmc2 (66531)
Gene Description:
COX assembly mitochondrial protein 2
Transcript:
RefSeq NM_026844.2 (NON-CURRENT)
Match location:
Position 169 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTAAAGGAATGTCACAA
Hairpin Sequence:
5'-CCGG-CCTGCTAAAGGAATGTCACAA-CTCGAG-TTGTGACATTCCTTTAGCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTAAAGGAATGTCACAACTCGAGTTGTGACATTCCTTTAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTAAAGGAATGTCACAACTCGAGTTGTGACATTCCTTTAGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000279084