Construct: shRNA TRCN0000181959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177616.2-2071s1c1
DNA Barcode:
GCAAGTGCAAGCCAATGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc157 (216516)
Gene Description:
coiled-coil domain containing 157
Transcript:
RefSeq NM_177616.2 (NON-CURRENT)
Match location:
Position 2071 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGTGCAAGCCAATGCTAT
Hairpin Sequence:
5'-CCGG-GCAAGTGCAAGCCAATGCTAT-CTCGAG-ATAGCATTGGCTTGCACTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGTGCAAGCCAATGCTATCTCGAGATAGCATTGGCTTGCACTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGTGCAAGCCAATGCTATCTCGAGATAGCATTGGCTTGCACTTGC-3'

Other clones with same target sequence:

(none)