Construct: shRNA TRCN0000181976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019457.1-1452s1c1
DNA Barcode:
CCAGACTTTGAGGATAACCCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrc6 (54562)
Gene Description:
leucine rich repeat containing 6 (testis)
Transcript:
RefSeq NM_019457.1 (NON-CURRENT)
Match location:
Position 1452 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGACTTTGAGGATAACCCA
Hairpin Sequence:
5'-CCGG-CCAGACTTTGAGGATAACCCA-CTCGAG-TGGGTTATCCTCAAAGTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGACTTTGAGGATAACCCACTCGAGTGGGTTATCCTCAAAGTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGACTTTGAGGATAACCCACTCGAGTGGGTTATCCTCAAAGTCTGG-3'

Other clones with same target sequence:

(none)