Construct: shRNA TRCN0000181977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026143.1-743s1c1
DNA Barcode:
CGTACATATACACCAAGGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Far1 (67420)
Gene Description:
fatty acyl CoA reductase 1
Transcript:
RefSeq NM_026143.1 (NON-CURRENT)
Match location:
Position 743 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTACATATACACCAAGGCAT
Hairpin Sequence:
5'-CCGG-CGTACATATACACCAAGGCAT-CTCGAG-ATGCCTTGGTGTATATGTACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTACATATACACCAAGGCATCTCGAGATGCCTTGGTGTATATGTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTACATATACACCAAGGCATCTCGAGATGCCTTGGTGTATATGTACG-3'

Other clones with same target sequence:

(none)