Construct: shRNA TRCN0000182005

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013806.1-739s1c1
DNA Barcode:
CCTAGAACTTCACTGGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankrd13c (433667)
Gene Description:
ankyrin repeat domain 13c
Transcript:
RefSeq NM_001013806.1 (CURRENT)
Match location:
Position 739 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182005)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGAACTTCACTGGGATTT
Hairpin Sequence:
5'-CCGG-CCTAGAACTTCACTGGGATTT-CTCGAG-AAATCCCAGTGAAGTTCTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGAACTTCACTGGGATTTCTCGAGAAATCCCAGTGAAGTTCTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGAACTTCACTGGGATTTCTCGAGAAATCCCAGTGAAGTTCTAGG-3'

Other clones with same target sequence:

(none)