Construct: shRNA TRCN0000182050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175380.3-1086s1c1
DNA Barcode:
CTGCAGTGTATCAGATCTGCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpd1l (333433)
Gene Description:
glycerol-3-phosphate dehydrogenase 1-like
Transcript:
RefSeq NM_175380.3 (NON-CURRENT)
Match location:
Position 1086 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCAGTGTATCAGATCTGCT
Hairpin Sequence:
5'-CCGG-CTGCAGTGTATCAGATCTGCT-CTCGAG-AGCAGATCTGATACACTGCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCAGTGTATCAGATCTGCTCTCGAGAGCAGATCTGATACACTGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCAGTGTATCAGATCTGCTCTCGAGAGCAGATCTGATACACTGCAG-3'

Other clones with same target sequence:

(none)