Construct: shRNA TRCN0000182107

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199197.1-793s1c1
DNA Barcode:
CGAGGCGCTGAATAAGCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbfa (68731)
Gene Description:
ribosome binding factor A
Transcript:
RefSeq NM_199197.1 (CURRENT)
Match location:
Position 793 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182107)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGCGCTGAATAAGCAAAT
Hairpin Sequence:
5'-CCGG-CGAGGCGCTGAATAAGCAAAT-CTCGAG-ATTTGCTTATTCAGCGCCTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGCGCTGAATAAGCAAATCTCGAGATTTGCTTATTCAGCGCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGCGCTGAATAAGCAAATCTCGAGATTTGCTTATTCAGCGCCTCG-3'

Other clones with same target sequence:

(none)