Construct: shRNA TRCN0000182126

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201374.1-1509s1c1
DNA Barcode:
CCCATCACTAGACTTGGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc155 (384619)
Gene Description:
coiled-coil domain containing 155
Transcript:
RefSeq NM_201374.1 (NON-CURRENT)
Match location:
Position 1509 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182126)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCACTAGACTTGGAGAT
Hairpin Sequence:
5'-CCGG-CCCATCACTAGACTTGGAGAT-CTCGAG-ATCTCCAAGTCTAGTGATGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCACTAGACTTGGAGATCTCGAGATCTCCAAGTCTAGTGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCACTAGACTTGGAGATCTCGAGATCTCCAAGTCTAGTGATGGG-3'

Other clones with same target sequence:

(none)