Construct: shRNA TRCN0000182171

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026034.1-327s1c1
DNA Barcode:
CAACCGACGATCCTGTCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Armc10 (67211)
Gene Description:
armadillo repeat containing 10
Transcript:
RefSeq NM_026034.1 (NON-CURRENT)
Match location:
Position 327 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182171)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCGACGATCCTGTCATTA
Hairpin Sequence:
5'-CCGG-CAACCGACGATCCTGTCATTA-CTCGAG-TAATGACAGGATCGTCGGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCGACGATCCTGTCATTACTCGAGTAATGACAGGATCGTCGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCGACGATCCTGTCATTACTCGAGTAATGACAGGATCGTCGGTTG-3'

Other clones with same target sequence:

(none)