Construct: shRNA TRCN0000182320
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001013829.1-470s1c1
- DNA Barcode:
- GAGTGGAAGAAGGAACGGATT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Shf (435684)
- Gene Description:
- Src homology 2 domain containing F
- Transcript:
- RefSeq NM_001013829.1 (NON-CURRENT)
- Match location:
- Position 470 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000182320)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAGTGGAAGAAGGAACGGATT
- Hairpin Sequence:
-
5'-CCGG-GAGTGGAAGAAGGAACGGATT-CTCGAG-AATCCGTTCCTTCTTCCACTC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAGTGGAAGAAGGAACGGATTCTCGAGAATCCGTTCCTTCTTCCACTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAGTGGAAGAAGGAACGGATTCTCGAGAATCCGTTCCTTCTTCCACTC-3'
Other clones with same target sequence:
(none)
