Construct: shRNA TRCN0000182328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018873.1-2789s1c1
DNA Barcode:
GCTGAAACCGACTTCAGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srcin1 (56013)
Gene Description:
SRC kinase signaling inhibitor 1
Transcript:
RefSeq NM_018873.1 (NON-CURRENT)
Match location:
Position 2789 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAACCGACTTCAGCAAA
Hairpin Sequence:
5'-CCGG-GCTGAAACCGACTTCAGCAAA-CTCGAG-TTTGCTGAAGTCGGTTTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAACCGACTTCAGCAAACTCGAGTTTGCTGAAGTCGGTTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAACCGACTTCAGCAAACTCGAGTTTGCTGAAGTCGGTTTCAGC-3'

Other clones with same target sequence:

(none)