Construct: shRNA TRCN0000182352

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198884.1-856s1c1
DNA Barcode:
GAGACCATCCTAAGGATGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B4galnt3 (330406)
Gene Description:
beta-1,4-N-acetyl-galactosaminyl transferase 3
Transcript:
RefSeq NM_198884.1 (CURRENT)
Match location:
Position 856 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182352)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACCATCCTAAGGATGGAT
Hairpin Sequence:
5'-CCGG-GAGACCATCCTAAGGATGGAT-CTCGAG-ATCCATCCTTAGGATGGTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACCATCCTAAGGATGGATCTCGAGATCCATCCTTAGGATGGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACCATCCTAAGGATGGATCTCGAGATCCATCCTTAGGATGGTCTC-3'

Other clones with same target sequence:

(none)