Construct: shRNA TRCN0000182414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009185.1-4403s1c1
DNA Barcode:
GCCGCAGCTAATCAAGTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stil (20460)
Gene Description:
Scl/Tal1 interrupting locus
Transcript:
RefSeq NM_009185.1 (NON-CURRENT)
Match location:
Position 4403 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCAGCTAATCAAGTTCAA
Hairpin Sequence:
5'-CCGG-GCCGCAGCTAATCAAGTTCAA-CTCGAG-TTGAACTTGATTAGCTGCGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCAGCTAATCAAGTTCAACTCGAGTTGAACTTGATTAGCTGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCAGCTAATCAAGTTCAACTCGAGTTGAACTTGATTAGCTGCGGC-3'

Other clones with same target sequence:

  1. TRCN0000328074