Construct: shRNA TRCN0000182450

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172496.2-328s1c1
DNA Barcode:
GCAATGATGGACCTACTGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cobl (12808)
Gene Description:
cordon-bleu WH2 repeat
Transcript:
RefSeq NM_172496.2 (NON-CURRENT)
Match location:
Position 328 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182450)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATGATGGACCTACTGGTT
Hairpin Sequence:
5'-CCGG-GCAATGATGGACCTACTGGTT-CTCGAG-AACCAGTAGGTCCATCATTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATGATGGACCTACTGGTTCTCGAGAACCAGTAGGTCCATCATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATGATGGACCTACTGGTTCTCGAGAACCAGTAGGTCCATCATTGC-3'

Other clones with same target sequence:

(none)