Construct: shRNA TRCN0000182512
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_028223.2-2114s1c1
- DNA Barcode:
- CCCATTCCTGTACCAGTTGTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Tmem175 (72392)
- Gene Description:
- transmembrane protein 175
- Transcript:
- RefSeq NM_028223.2 (NON-CURRENT)
- Match location:
- Position 2114 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000182512)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCCATTCCTGTACCAGTTGTT
- Hairpin Sequence:
-
5'-CCGG-CCCATTCCTGTACCAGTTGTT-CTCGAG-AACAACTGGTACAGGAATGGG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCCATTCCTGTACCAGTTGTTCTCGAGAACAACTGGTACAGGAATGGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCCATTCCTGTACCAGTTGTTCTCGAGAACAACTGGTACAGGAATGGG-3'
Other clones with same target sequence:
(none)
