Construct: shRNA TRCN0000182517

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176923.2-2732s1c1
DNA Barcode:
CCTCACAAAGGGATCGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr59 (319481)
Gene Description:
WD repeat domain 59
Transcript:
RefSeq NM_176923.2 (NON-CURRENT)
Match location:
Position 2732 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182517)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCACAAAGGGATCGAGTTT
Hairpin Sequence:
5'-CCGG-CCTCACAAAGGGATCGAGTTT-CTCGAG-AAACTCGATCCCTTTGTGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCACAAAGGGATCGAGTTTCTCGAGAAACTCGATCCCTTTGTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCACAAAGGGATCGAGTTTCTCGAGAAACTCGATCCCTTTGTGAGG-3'

Other clones with same target sequence:

(none)