Construct: shRNA TRCN0000182535

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172737.1-1124s1c1
DNA Barcode:
GCAAGAACGAAGTGACTGTCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shisa7 (232813)
Gene Description:
shisa family member 7
Transcript:
RefSeq NM_172737.1 (NON-CURRENT)
Match location:
Position 1124 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182535)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGAACGAAGTGACTGTCT
Hairpin Sequence:
5'-CCGG-GCAAGAACGAAGTGACTGTCT-CTCGAG-AGACAGTCACTTCGTTCTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGAACGAAGTGACTGTCTCTCGAGAGACAGTCACTTCGTTCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGAACGAAGTGACTGTCTCTCGAGAGACAGTCACTTCGTTCTTGC-3'

Other clones with same target sequence:

(none)