Construct: shRNA TRCN0000182538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178614.2-258s1c1
DNA Barcode:
GTGGTGGTTCAGCATGTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Samm50 (68653)
Gene Description:
SAMM50 sorting and assembly machinery component
Transcript:
RefSeq NM_178614.2 (NON-CURRENT)
Match location:
Position 258 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGTGGTTCAGCATGTTCAT
Hairpin Sequence:
5'-CCGG-GTGGTGGTTCAGCATGTTCAT-CTCGAG-ATGAACATGCTGAACCACCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGTGGTTCAGCATGTTCATCTCGAGATGAACATGCTGAACCACCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGTGGTTCAGCATGTTCATCTCGAGATGAACATGCTGAACCACCAC-3'

Other clones with same target sequence:

  1. TRCN0000346083