Construct: shRNA TRCN0000182610

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023268.1-1451s1c1
DNA Barcode:
GACTAGCCACAACAGGGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Qsox1 (104009)
Gene Description:
quiescin Q6 sulfhydryl oxidase 1
Transcript:
RefSeq NM_023268.1 (NON-CURRENT)
Match location:
Position 1451 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182610)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTAGCCACAACAGGGTTAA
Hairpin Sequence:
5'-CCGG-GACTAGCCACAACAGGGTTAA-CTCGAG-TTAACCCTGTTGTGGCTAGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTAGCCACAACAGGGTTAACTCGAGTTAACCCTGTTGTGGCTAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTAGCCACAACAGGGTTAACTCGAGTTAACCCTGTTGTGGCTAGTC-3'

Other clones with same target sequence:

(none)