Construct: shRNA TRCN0000182614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178113.2-2731s1c1
DNA Barcode:
GCTGTGTGTCATTGGGCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ncapd3 (78658)
Gene Description:
non-SMC condensin II complex, subunit D3
Transcript:
RefSeq NM_178113.2 (NON-CURRENT)
Match location:
Position 2731 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGTGTCATTGGGCATAT
Hairpin Sequence:
5'-CCGG-GCTGTGTGTCATTGGGCATAT-CTCGAG-ATATGCCCAATGACACACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGTGTCATTGGGCATATCTCGAGATATGCCCAATGACACACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGTGTCATTGGGCATATCTCGAGATATGCCCAATGACACACAGC-3'

Other clones with same target sequence:

(none)