Construct: shRNA TRCN0000182618

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007508.2-1833s1c1
DNA Barcode:
GCAAAGATCAAGGCCGACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v1a (11964)
Gene Description:
ATPase, H+ transporting, lysosomal V1 subunit A
Transcript:
RefSeq NM_007508.2 (NON-CURRENT)
Match location:
Position 1833 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182618)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGATCAAGGCCGACTAT
Hairpin Sequence:
5'-CCGG-GCAAAGATCAAGGCCGACTAT-CTCGAG-ATAGTCGGCCTTGATCTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGATCAAGGCCGACTATCTCGAGATAGTCGGCCTTGATCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGATCAAGGCCGACTATCTCGAGATAGTCGGCCTTGATCTTTGC-3'

Other clones with same target sequence:

(none)