Construct: shRNA TRCN0000182692

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011955.1-257s1c1
DNA Barcode:
GAGGATGGAGATACACAGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nubp1 (26425)
Gene Description:
nucleotide binding protein 1
Transcript:
RefSeq NM_011955.1 (NON-CURRENT)
Match location:
Position 257 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182692)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGATGGAGATACACAGGTT
Hairpin Sequence:
5'-CCGG-GAGGATGGAGATACACAGGTT-CTCGAG-AACCTGTGTATCTCCATCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGATGGAGATACACAGGTTCTCGAGAACCTGTGTATCTCCATCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGATGGAGATACACAGGTTCTCGAGAACCTGTGTATCTCCATCCTC-3'

Other clones with same target sequence:

  1. TRCN0000292558