Construct: shRNA TRCN0000182819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013768.1-2005s1c1
DNA Barcode:
CAGCTCTGAGTTCTCTTCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prmt5 (27374)
Gene Description:
protein arginine N-methyltransferase 5
Transcript:
RefSeq NM_013768.1 (NON-CURRENT)
Match location:
Position 2005 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTCTGAGTTCTCTTCCTA
Hairpin Sequence:
5'-CCGG-CAGCTCTGAGTTCTCTTCCTA-CTCGAG-TAGGAAGAGAACTCAGAGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTCTGAGTTCTCTTCCTACTCGAGTAGGAAGAGAACTCAGAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTCTGAGTTCTCTTCCTACTCGAGTAGGAAGAGAACTCAGAGCTG-3'

Other clones with same target sequence:

(none)