Construct: shRNA TRCN0000182869

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013534.3-1138s1c1
DNA Barcode:
CTCGGAGAGAAGAGACAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
P3h3 (14789)
Gene Description:
prolyl 3-hydroxylase 3
Transcript:
RefSeq NM_013534.3 (NON-CURRENT)
Match location:
Position 1138 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182869)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGGAGAGAAGAGACAGTTA
Hairpin Sequence:
5'-CCGG-CTCGGAGAGAAGAGACAGTTA-CTCGAG-TAACTGTCTCTTCTCTCCGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGGAGAGAAGAGACAGTTACTCGAGTAACTGTCTCTTCTCTCCGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGGAGAGAAGAGACAGTTACTCGAGTAACTGTCTCTTCTCTCCGAG-3'

Other clones with same target sequence:

  1. TRCN0000319994