Construct: shRNA TRCN0000182883

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004666.1-594s1c1
DNA Barcode:
CCTGAGATTGTGACTTTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VNN1 (8876)
Gene Description:
vanin 1
Transcript:
RefSeq NM_004666.1 (NON-CURRENT)
Match location:
Position 594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182883)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGATTGTGACTTTCAAT
Hairpin Sequence:
5'-CCGG-CCTGAGATTGTGACTTTCAAT-CTCGAG-ATTGAAAGTCACAATCTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGATTGTGACTTTCAATCTCGAGATTGAAAGTCACAATCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGATTGTGACTTTCAATCTCGAGATTGAAAGTCACAATCTCAGG-3'

Other clones with same target sequence:

(none)