Construct: shRNA TRCN0000182891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028790.1-1174s1c1
DNA Barcode:
CCATATCTGTTAAGGTTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acot12 (74156)
Gene Description:
acyl-CoA thioesterase 12
Transcript:
RefSeq NM_028790.1 (NON-CURRENT)
Match location:
Position 1174 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATATCTGTTAAGGTTGAAA
Hairpin Sequence:
5'-CCGG-CCATATCTGTTAAGGTTGAAA-CTCGAG-TTTCAACCTTAACAGATATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATATCTGTTAAGGTTGAAACTCGAGTTTCAACCTTAACAGATATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATATCTGTTAAGGTTGAAACTCGAGTTTCAACCTTAACAGATATGG-3'

Other clones with same target sequence:

(none)