Construct: shRNA TRCN0000182983

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177171.3-6706s1c1
DNA Barcode:
CGTATCAAACTGTGTTGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Heatr5a (320487)
Gene Description:
HEAT repeat containing 5A
Transcript:
RefSeq NM_177171.3 (NON-CURRENT)
Match location:
Position 6706 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182983)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTATCAAACTGTGTTGCATT
Hairpin Sequence:
5'-CCGG-CGTATCAAACTGTGTTGCATT-CTCGAG-AATGCAACACAGTTTGATACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTATCAAACTGTGTTGCATTCTCGAGAATGCAACACAGTTTGATACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTATCAAACTGTGTTGCATTCTCGAGAATGCAACACAGTTTGATACG-3'

Other clones with same target sequence:

(none)