Construct: shRNA TRCN0000183135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175672.2-190s1c1
DNA Barcode:
CCGGAACATATTTACAATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4930579C12Rik (319213)
Gene Description:
RIKEN cDNA 4930579C12 gene
Transcript:
RefSeq NM_175672.2 (NON-CURRENT)
Match location:
Position 190 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAACATATTTACAATCAT
Hairpin Sequence:
5'-CCGG-CCGGAACATATTTACAATCAT-CTCGAG-ATGATTGTAAATATGTTCCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAACATATTTACAATCATCTCGAGATGATTGTAAATATGTTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAACATATTTACAATCATCTCGAGATGATTGTAAATATGTTCCGG-3'

Other clones with same target sequence:

(none)